Overcoming challenges in development of LC/MS/MS methods for a highly sulfated carbohydrate, sucrose octasulfate in biological matrices

Posted April 14, 2015

By: Dr. Theo Kapanadze, D.Sc., Ph.D.

Polysulfated carbohydrates are an important class of com-pounds in terms of biological activity and pharmaceuticalimportance. For example, heparin has been found to be involved in many biological processes such as blood coagulation, inflammation and even cancer disease.

Sucrose octasulfate is a syntheticpolysulfated carbohydrate. An aluminum salt of sucrose octasul-fate (SOS) is clinically used in the treatment of duodenal ulcers. It involves the stabilization of fibroblast growth factor, thus promoting wound healing.

New and important applications of sucroseoctasulfate and its analogs in wound healing and in the treatment of cancer have been suggested from some studies. However, there are a number of challenges for the quantitative determination of sucrose octasulfate and its polysulfated carbohydrates family in biological matrix due to the lack of means of sensitive detection.

In short, their mass spectrum analysis is every challenging.

In the present study, a simple, sensitive and rapid LC/MS/MS method for the quantitation of SOS in biological matrix is described. To author's knowledge, this is the first method ever reported for the quantitative analysis of sucrose octasulfate in biological samples using LC–MS/MS.

Read the full-text article, entitled "A novel ultra performance liquid chromatography–tandem mass spectrometry method for the determination of sucrose octasulfate in dog plasma", published in the Journal of Chromatography B.

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